Author(s): Fekner T, Li X, Lee MM, Chan MK
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Abstract Ignoring the STOP sign: A pyrrolysine analogue bearing a terminal alkyne was site-specifically incorporated into recombinant calmodulin (CaM) through a UAG codon. The resulting protein was labeled with an azide-containing dye using a copper(I)-catalyzed click reaction. Subsequent application of an orthogonal cysteine tagging method yielded a CaM labeled with two distinct fluorophores that enabled its study by FRET spectroscopy.
This article was published in Angew Chem Int Ed Engl
and referenced in Organic Chemistry: Current Research