alexa A quantitative thin layer chromatography method for determination of theophylline in plasma.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Mirfazaelian A, Goudarzi M, Tabatabaiefar M, Mahmoudian M

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Abstract A simple assay method for theophylline in plasma using thin layer chromatography (TLC) was developed. The method involves extraction of the drug and internal standard (acetaminophen) by chloroform-isopropanol (75:25) followed by separation on TLC silica plates using a mixture of acetic acid, isopropanol, toluene (1: 12: 6), as the eluting solvent. Both peak height ratios and peak are ratios showed high correlation coefficient (r>0.98, p<0.001). However we used peak heights for the determinations. Within-day and between-day coefficients of variation were less than 4.4\% and 7.8\% respectively. The assay proved inexpensive, accurate and reproducible with a limit of detection of 100 ng/ml that makes it suitable for bioavailability studies.
This article was published in J Pharm Pharm Sci and referenced in Journal of Chromatography & Separation Techniques

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