alexa A sensitive high-throughput HPLC assay for simultaneous determination of everolimus and clobetasol propionate.
Biomedical Sciences

Biomedical Sciences

Journal of Bioanalysis & Biomedicine

Author(s): Kamberi M, Fu K, Lu J, Chemaly GM, Feder D

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Abstract A novel sensitive high-throughput high-performance liquid chromatography assay is developed and validated for the simultaneous determination of everolimus and clobetasol propionate in pharmaceutical formulations. The chromatographic separation is achieved on a Zorbax Eclipse XDB-C18 reversed-phase column using a gradient elution, with solvent A: ammonium acetate (pH 6.8; 0.01 M) and solvent B: acetonitrile. The mean recovery ranges from 95.1\% to 100.0\% for clobetasol propionate and from 97.9\% to 103.7\% for everolimus. The limit of quantitation for each analyte is 0.02 microg/mL. The percent relative standard deviations are less than 3\% for intra- and inter-day analyses. The proposed method can be used for the routine quality control of everolimus and clobetasol propionate in complex pharmaceutical formulations, especially the drug-delivery systems with a low total drug-load.
This article was published in J Chromatogr Sci and referenced in Journal of Bioanalysis & Biomedicine

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