Author(s): Rae JL, Truitt KD, Kuszak JR
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Abstract Lenses fixed in formaldehyde or glutaraldehyde can be embedded in plastic, and sections as large as 3-4 mm across can be cut with glass knives on an ultramicrotome. When these sections are stained with 25\% sodium fluorescein and allowed to bleach in a fluorescence microscope, high contrast images of essentially every cell in the section, including cells very near the lens center, can be seen. The method is effective with lenses from a number of species, and should be useful for a wide variety of morphological studies of normal and cataractous lenses.
This article was published in Curr Eye Res
and referenced in Journal of Clinical & Experimental Ophthalmology