alexa A simple hydrogen peroxide biosensor based on a novel electro-magnetic poly(p-phenylenediamine)@Fe3O4 nanocomposite.
Engineering

Engineering

Journal of Biosensors & Bioelectronics

Author(s): Baghayeri M, Nazarzadeh Zare E, Mansour Lakouraj M

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Abstract The novel biocompatible poly(p-phenylenediamine) (PpPDA)-Fe3O4 nanocomposite ([email protected]) was synthesized via emulsion polymerization. The [email protected] nanocomposite was characterized by Fourier transform infrared spectra (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and vibrating sample magnetometer (VSM). The [email protected] nanocomposite was then used as substrate for the immobilization of hemoglobin (Hb) and their bioelectrochemical behaviors were studied. Electrochemical impedance spectroscopy was used to confirm the adsorption of Hb onto the surface of [email protected] nanocomposite. The Hb immobilized on [email protected] nanocomposite retained its near-native conformations as characterized by the FT-IR. A pair of well-defined redox peaks of Hb was obtained at the [email protected] modified glassy carbon electrode ([email protected]/GCE) through direct electron transfer between the protein and the underlying electrode. The proposed biosensor showed good reproducibility and high sensitivity to H2O2 with the detection limit of 0.21 µM (S/N=3). In the range of 0.5-400.0 µM, the catalytic reduction current of H2O2 was proportional to its concentration. The apparent Michaelis-Menten constant of Hb on the [email protected] nanocomposite was estimated to be 0.088 mM, showing its high affinity. Copyright © 2014 Elsevier B.V. All rights reserved. This article was published in Biosens Bioelectron and referenced in Journal of Biosensors & Bioelectronics

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