Author(s): Nishihara T, Iwabuchi T, Nohno T
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Abstract An expression vector, pKK233-2 [Amann and Brosius, Gene 40 (1985) 183-190], was modified by replacing the trc promoter with the T7 phi 10 promoter and introducing a strong transcriptional terminator upstream from this promoter. This modification successfully suppressed background transcription and resulted in strict dependence upon induction.
This article was published in Gene
and referenced in Journal of Cell Science & Therapy