alexa Activation of the Epstein-Barr virus genome by 5-aza-cytidine in latently infected human lymphoid lines.
Chemistry

Chemistry

Medicinal chemistry

Author(s): BenSasson SA, Klein G

Abstract Share this page

Abstract Recent studies indicate that gene expression in higher eukaryotes is accompanied by a decrease in the frequency of 5-methyl cytosine residues around the activated site (Razin and Riggs, 1980). 5-aza-cytidine (5-aza-C) is an analogue that reduces cytidine methylation in DNA (Jones and Taylor, 1980) and has been reported to change the differentiation pattern of cultured mouse embryo cells (Taylor and Jones, 1979). We have tested its ability to activate the Epstein-Barr virus cycle in latently EBV-infected human lymphoid lines. After an incubation period of 6 to 8 h with the drug, early antigens (EA) were induced in a substantial fraction of the cells in all six lines tested that had a low rate of spontaneous viral antigen production. Optimal conditions for EA induction were defined. The efficiency of 5-aza-C was comparable to the inducing effect of iododeoxyuridine. EBV-DNA and EBNA positive virus-non-producer lines did not respond to 5-aza-C treatment. The findings are discussed in relation to the possibility that changes in EBV-gene expression may be related to the state of DNA methylation.
This article was published in Int J Cancer and referenced in Medicinal chemistry

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords