Author(s): Nicholson CA, Fathepure BZ
Abstract Share this page
Abstract The Great Salt Plains is a 65-km(2) hypersaline habitat of geological origin located in north-central Oklahoma. Contamination of such ecosystems by petroleum compounds is expected from non-point sources and due to increased human activities. Little information exists about the ability of halophilic and halotolerant bacteria present in such ancient and uncontaminated environments to degrade aromatic hydrocarbons. An enrichment culture was established from soil samples obtained from the salt flats using benzene as the sole carbon and energy source. The enrichment degraded benzene at varied salt concentrations ranging from 0 to 4M. Studies showed that roughly 33\% of the (14)C-benzene was converted to (14)CO(2), indicating the mineralization capacity of native bacteria. Bacterial community structure analysis using denaturing gradient gel electrophoresis showed that different phylotypes were dominant at different salt concentrations.
This article was published in FEMS Microbiol Lett
and referenced in Journal of Physical Chemistry & Biophysics