alexa Alginate microbeads as inoculant carriers for plant growth-promoting bacteria
Agri and Aquaculture

Agri and Aquaculture

Journal of Agricultural Science and Food Research

Author(s): Yoav Bashan, JuanPablo Hernandez, Luis A Leyva, Macario Bacilio

Abstract Share this page

A method of inoculating wet and dry seeds with plant growth-promoting bacteria (PGPB) using alginate microbeads as a substrate and Azospirillum brasilense as the model PGPB was developed. The microbeads were produced by low pressure spraying of an alginate solution mixed with liquid bacterial culture suspended in a very rich medium through a small nozzle resulting in small-diameter droplets. These droplets, when sprayed into a slowly stirred solution of CaCl2, immediately hardened into microbeads at diameters ranging between 100 and 200 µm. Although the process killed part of the entrapped bacteria, the remaining bacteria residing in the microbeads were sufficient [>1011 colony-forming units (CFU) g–1 inoculant] for seed inoculation. Further, it was found that the bacterial population in the inoculant could be enhanced by secondary multiplication in the same medium for an additional 16 h. It was found that the microbeads could be used either wet or dry. Dry inoculant was produced using dry air at 38°C, creating a powdery substance loaded with >109 CFU g–1 beads. Alternatively, dry microbeads were produced using a standard freeze-drying procedure. This dry preparation was easily attached to dry seed surfaces with the addition of 1% alcohol-diluted lecithin or with 0.5% synthetic paper adhesive (Resistol). The bacteria were slowly released from the microbeads in amounts ranging from 104 to 106 CFU g–1 depending on the type (wet or dry, with or without skim milk) and the time of incubation (the longer the incubation period, the smaller the amount of bacteria released with time). The wet and dry inoculants enhanced the development of wheat and tomato seedlings growing in unfertile soil, and biodegraded within 15 days in moist soil.

This article was published in Biology and Fertility of Soils and referenced in Journal of Agricultural Science and Food Research

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords