alexa An alternative PCR assay for quantifying mitochondrial DNA in crude preparations.
Immunology

Immunology

Immunome Research

Author(s): Koekemoer TC, Downing TG, Oelofsen W, Koekemoer TC, Downing TG, Oelofsen W

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Abstract A method is described for the quantification of mitochondrial DNA present in crude biological preparations. A known copy number of a standard is amplified in the presence of inactivated target DNA so as to determine the overall efficiency of the PCR process in a particular sample. In this way any inhibitory and/or stimulatory substances present in sample preparations can be taken into account. To reduce tube-to-tube variations product DNA quantification is limited to small cycle numbers. Using this method quantitations of DNA amounts in different crude preparations can be compared.
This article was published in Nucleic Acids Res and referenced in Immunome Research

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