alexa An improved method for the purification and refolding of r56-kDa proteins from Gilliam and Kato strains of Orientia tsutsugamushi.


Journal of Vaccines & Vaccination

Author(s): Yang Q, Ching WM, Jiang J, Lousteau L, Richards AL

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Abstract The immunodominant 56kDa outer membrane antigens from Orientia tsutsugamushi Kato and Gilliam strains were expressed as inclusion bodies (IBs) in E. coli. The IBs were purified and properly refolded with modifications of a previous procedure used for the production of Karp strain r56 antigen. A mixture of these three r56 proteins exhibited both high sensitivity and specificity for detection of O. tsutsugamushi antibodies by ELISA.
This article was published in Ann N Y Acad Sci and referenced in Journal of Vaccines & Vaccination

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