alexa An In Vitro Study of the Reaction of Periodontal and Gingival Fibroblasts to Low-level Laser Irradiation: A Pilot Study


Journal of Lasers, Optics & Photonics

Author(s): Angela Dominguez Camacho

Abstract Share this page

ABSTRACTPurpose: To evaluate the effect of laser irradiation on cell proliferation of periodontal and gingival fibroblasts with a low energy level laser. Materials and Methods: Periodontal and gingival fibroblast cultures were obtained from the tissue bank of the Universidad del Valle (Cali, Colombia). After 24 h of incubation, they were irradiated while in the logarithmic phase of growth, using a low energy level laser (Photon LASE As-Ga-Al), operated at 37 mW, 830 nm wavelength at an energy level of 3.75 J/cm2. The rate of cell proliferation was evaluated in experimental and control cultures by the XTT test (Roche). The percentage of cytotoxicity was measured by the LDH test (Roche). The rate of proliferation was measured 24, 48, 72, 96, 120, and 144 h after irradiation. The cell cytotoxicity was measured 24 h after irradiation. Results: The irradiation protocol used in this study did not cause cytotoxicity in any of the cell lines evaluated. The rate of gingival fibroblast proliferation was higher in the experimental culture than in the controls, although the difference was not statistically significant, while for periodontal fibroblasts the rate of cell proliferation was higher in the control group, but not significantly differently. Conclusion: The cytoxicity test indicates that this laser treatment protocol is safe for both cell lines. There are no statistically significant differences in the rate of cell proliferation between or within groups.

  • To read the full article Visit
  • Open Access
This article was published in Academic Journal and referenced in Journal of Lasers, Optics & Photonics

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 6th International Conference on Photonics
    July 31- August 01, 2017 Milan, Italy
  • 7th International Conference on Laser Optics
    July 31- August 02, 2017 Milan,Italy
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version