alexa AN INNOVATIVE DERIVATIZATION METHOD FOR SIMULTANEOUS DETERMINATION OF URONIC ACIDS AND NEUTRAL AND AMINO SUGARS IN COEXISTING SAMPLES BY HPLC-ESI-MS MS
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Ying Zhang, Ping Zhang, Zhongfu Wang, Linjuan Huang

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To eliminate lactone byproduct of uronic acid and avoid a desalting procedure, a novel strategy of saccharides derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was developed for analysis of uronic acids and neutral and amino sugars in coexisting samples by HPLC-ESI-MS, initially employing triethylamine as a catalyst instead of the conventional sodium hydroxide. The established method was greatly simplified compared to conventional methods, and the derivatives could be directly analyzed by ESI-MS without a tedious desalting procedure, avoiding loss of the sample. The assay showed excellent linearity over the range 0.025–1.60 µmol L−1 (r > 0.999) and detection limits ranging from 0.01 to 0.09 pmol. The intra-day precision was found to be satisfactory (RSD <2.7%). Mean recoveries ranged from 93.74% to 104.23%. Thirteen PMP-labeled monosaccharides including uronic acids and neutral and amino sugars were baseline separated on a C18 column and detected by HPLC-ESI-MS/MS2 within only 22 min. The assay was successfully applied to sugar composition analysis of polysaccharides ASP from Artemisia sphaerocephala Krasch.

This article was published in Journal of Liquid Chromatography & Related Technologies and referenced in Journal of Chromatography & Separation Techniques

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