alexa An integrated culture and real-time PCR method to assess viability of disinfectant treated Bacillus spores using robotics and the MPN quantification method.
General Science

General Science

Journal of Bioterrorism & Biodefense

Author(s): Varughese EA, Wymer LJ, Haugland RA

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Abstract Using robotics and the MPN technique, a 96-microwell method was developed to compare two procedures for enumeration of viable chlorine-treated B. atrophaeus spores: broth-culture enrichment followed by real-time polymerase chain reaction analysis; and filter plating on agar. Recoveries of chlorine-treated spores were improved by broth enrichment over filter plating, whereas recoveries of non-treated spores were not different in the two procedures. This article was published in J Microbiol Methods and referenced in Journal of Bioterrorism & Biodefense

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