alexa An integrated giant magnetoimpedance biosensor for detection of biomarker.
Materials Science

Materials Science

Journal of Nanomedicine & Nanotechnology

Author(s): Wang T, Yang Z, Lei C, Lei J, Zhou Y

Abstract Share this page

Abstract A Dynabeads-labeled magnetic immunoassay (MIA) has been developed by using an integrated giant magnetoimpedance (GMI) biosensor for the detection of alpha-fetoprotein (AFP). The GMI biosensor (Cr/Cu/NiFe/Cu/NiFe/Al2O3/Cr/Au films) integrated magnetic sensing elements and a biomolecular immunoplatform. Au film was modified with 11-Mercaptoundecanoic acid (11-MUA) for the immobilization of AFP monoclonal antibody. Double antibody sandwich immunoassay was used to specifically capture and label AFP antigen. Functionalized Dynabeads were conjugated to AFP antigen by streptavidin-biotin binding assay. GMI responses were measured for sensitive detection of AFP from 1 to 10ng/ml. Our results revealed that the presence of AFP on the biosensor improved the GMI effect owing to the induced magnetic dipole of superparamagnetic Dynabeads, and the GMI ratio was greatly increased at high frequency. Specificity of MIA was tested through the use of 1\% bovine serum albumin (BSA). The underlying biophysical mechanisms responsible for the enhanced GMI effect in the detection of AFP were discussed. This work provides a complex lab-on-chip MIA for the detection of biomarker, which may open up a new way for the development of GMI-based MIA in clinical trials. Copyright © 2014 Elsevier B.V. All rights reserved. This article was published in Biosens Bioelectron and referenced in Journal of Nanomedicine & Nanotechnology

Relevant Expert PPTs

Recommended Conferences

  • Nano Congress for Next Generation
    August 31-September 01, 2017 Brussels,Belgium
  • Graphene & 2D Materials
    September 14-15, 2017 Edinburgh, Scotland
  • Graphene & 2D Materials
    November 6-7, 2017 Frankfurt, Germany
  • World Congress on Nanoscience and Nano Technology
    October 16-17, 2017 Dubai, UAE
  • World Medical Nanotechnology Congress
    October 18-19, 2017 Osaka, Japan
  • Nanoscienceand Molecular Nanotechnology
    Nov 06-08, 2017 Frankfurt, Germany
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version