alexa An ultra-performance liquid chromatography-tandem mass spectrometry method for determination of microcystins occurrence in surface water in Zhejiang Province, China.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Wang J, Pang X, Ge F, Ma Z

Abstract Share this page

Abstract A novel method had been developed for determination of microcystin in surface water by ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). Water samples after being concentrated and cleaned with solid phase extraction (SPE) with Oasis HLB cartridge were separated on AcQuity UPLC BEH C18 column (1.7 microm, 1.0 x 50 mm) with a gradient elution and a mobile phase consisting of 0.1\% formic acid in water and methanol, and then detected with an electrospray ionization tandem mass spectrometry in positive ion mode with multiple reaction monitoring. Compared with traditional liquid chromatography, it took much less time for UPLC to analyze microcystin. Additionally, the UPLC-MS/MS method produced satisfactory reliability, sensitivity, and accuracy. Recoveries of the four microcystins were 91.7-111\% with relative standard deviation being 7.9-12\%. The calibration curves for microcystins were linear with correlation coefficient being larger than 0.99. Limit of quantification of the four microcystins (microcystin-LR, RR, LW, and LF) after 1000-fold SPE pre-concentration, were 2.5, 6.0, 2.5, and 1.3 ng/L. The presented method had been successfully used to detect microcystin in surface water from the Qiantang River, the West Lake, and three drinking water reservoirs, which represented water bodies of river, lake, and drinking water reservoir, respectively. It was shown that microcystin pollution in drinking water reservoirs was the heaviest, and microcystin-LR and RR were the predominant kinds, with concentrations ranging 0.045-2.73 and 0.021-1.36 microg/L, respectively. The concentrations of microcystins in river and lake were all under detection limit. This article was published in Toxicon and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords