alexa Analysis of phospolipids and glycolipids by thin-layer chromatography-matrix-assisted laser desorption and ionization mass spectrometry.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Fuchs B

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Abstract Thin-layer chromatography-matrix-assisted laser desorption and ionization mass spectrometry (TLC-MALDI-MS) of organic extracts from biological samples allows untargeted analysis and structural characterization of phospholipids and glycolipids ionized from the near-surface region of a sample separated on a TLC-plate. In particular, MALDI-MS enables the sensitive detection of many analytes directly from the solid surface of an ordinary TLC-plate even without previous staining. It will be shown that the detailed fatty acyl composition of phospholipids can be determined solely by TLC-MALDI-MS without previous derivatization, enzymatic cleavage and/or reversed phase separation. MALDI-time-of-flight (TOF) MS is thus a powerful method in this field due to its high sensitivity, low extent of induced fragmentation and simple, user-friendly performance. This review summarizes the so far available knowledge about combined TLC-MALDI-MS for phospholipid and glycolipid characterization together with the technical workflow and a survey of applications. Finally a perspective on the future of TLC-MALDI-MS is given. Copyright © 2012 Elsevier B.V. All rights reserved.

This article was published in J Chromatogr A and referenced in Journal of Glycomics & Lipidomics

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