alexa Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Proteomics & Bioinformatics

Author(s): Mann M, Ong SE, Grnborg M, Steen H, Jensen ON,

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Abstract In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand signaling processes, we must first acquire an inventory of phosphoproteins and their phosphorylation sites under different conditions. Because phosphorylation is a dynamic process, elucidation of signaling networks also requires quantitation of these phosphorylation events. In this article, we outline several methods for enrichment of phosphorylated proteins and peptides and discuss various options for their identification and quantitation with special emphasis on mass spectrometry-based techniques.
This article was published in Trends Biotechnol and referenced in Journal of Proteomics & Bioinformatics

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