alexa Analytical methods for the determination of selected steroid sex hormones and corticosteriods in wastewater.
Toxicology

Toxicology

Journal of Environmental & Analytical Toxicology

Author(s): Vulliet E, Baugros JB, FlamentWaton MM, GrenierLoustalot MF, Vulliet E, Baugros JB, FlamentWaton MM, GrenierLoustalot MF

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Abstract An analytical method for the determination of 12 selected estrogens, progestagens and corticosteroids is presented. The optimization of the method, including liquid chromatography separation, extraction on a solid phase, purification on a silica gel cartridge and detection by mass spectrometry, is described. Both the repeatability, with relative standard deviation ranging from 1.4 to 2.7\%, and the accuracy, with recoveries ranging from 92.7 to 102.4\%, were very satisfactory for ten of the target analytes. The limits of detection were lower than 1 ng/L for progestagens, androgens and corticosteroids, and ranged between 0.9 and 4.3 ng/L for estrogens. The results of the analysis of two sewage treatment plants in the area of Lyon (France) by this method reveal that all the compounds investigated are present in the effluents. The estrogen most frequently detected was estrone, with a median of 26.1 ng/L. The target progestagens were detected with concentrations ranging between 5 and 41 ng/L. Androgens were also present in most of the samples in the range 1-30 ng/L, while the corticosteroids were present only in one plant, with a median of 31.9 ng/L. This article was published in Anal Bioanal Chem and referenced in Journal of Environmental & Analytical Toxicology

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