alexa Analyzing the cancer methylome through targeted bisulfite sequencing.
Genetics & Molecular Biology

Genetics & Molecular Biology

Gene Technology

Author(s): Lee EJ, Luo J, Wilson JM, Shi H

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Abstract Bisulfite conversion of genomic DNA combined with next-generation sequencing (NGS) has become a very effective approach for mapping the whole-genome and sub-genome wide DNA methylation landscapes. However, whole methylome shotgun bisulfite sequencing is still expensive and not suitable for analyzing large numbers of human cancer specimens. Recent advances in the development of targeted bisulfite sequencing approaches offer several attractive alternatives. The characteristics and applications of these methods are discussed in this review article. In addition, the bioinformatic tools that can be used for sequence capture probe design as well as downstream sequence analyses are also addressed. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
This article was published in Cancer Lett and referenced in Gene Technology

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