alexa Anti-apoptotic effect of acetyl-l-carnitine and I-carnitine in primary cultured neurons.
Psychiatry

Psychiatry

Journal of Addiction Research & Therapy

Author(s): Ishii T, Shimpo Y, Matsuoka Y, Kinoshita K

Abstract Share this page

Abstract Although exogenously administered acetyl-l-carnitine (ALCAR, (2-acetoxy-3-carboxypropyl)-trimethylammonium) and l-carnitine (LC, (3-carboxy-2-hydroxypropyl)-trimethylammonium) prevent brain damage in several ischemic models, the protective mechanism of these compounds remains unclear. Here, we evaluated the effect of ALCAR and LC in primary cultured neurons from the cerebral cortex, striatum and thalamus of 18-day-old rat embryos. Deprivation of the serum from cultured medium for 3 days reduced the number of viable cells and mitochondrial activity and induced cell death with characteristics of apoptosis such as DNA fragmentation, nuclear condensation and histone-DNA release into the cytoplasm. ALCAR (1 - 100 microM) and LC (1 - 100 microM) promoted neuronal survival and mitochondrial activity in a concentration-dependent manner. Moreover, these compounds attenuated DNA fragmentation and nuclear condensation in cultured neurons and significantly decreased histone-DNA release into the cytoplasm. These results indicate that anti-apoptotic actions of ALCAR and LC contribute to their neuroprotective effect.
This article was published in Jpn J Pharmacol and referenced in Journal of Addiction Research & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords