alexa Antibacterial action of chitosan and carboxymethylated chitosan
Environmental Sciences

Environmental Sciences

Hydrology: Current Research

Author(s): Xiao Fei Liu, Yun Lin Guan, Dong Zhi Yang, Zhi Li, Kang De Yao

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A series of chitosan with different molecular weights obtained by γ-irradiation depolymerization and another series of deacetylated chitosan were synthesized. Several N,O-carboxymethylated chitosan and O-carboxymethylated chitosan were also produced. The above samples were characterized by Fourier transform infrared spectroscopy (FTIR). Their antibacterial activities against E. coli were explored by the optical density method. The antibacterial activity of chitosan is influenced by its molecular weight, degree of deacetylation, concentration in solution, and pH of the medium. Antibacterial activities were also found to be increased in the order of N,O-carboxymethylated chitosan, chitosan, and O-carboxymethylated chitosan. Fluorescence of the FITC (fluorescein isothiocyanate)-labeled chitosan oligomers at the inside of the E. coli cell was observed by a confocal laser scanning microscope. The antibacterial activity of chitosan oligomers seems to be caused mainly by the inhibition of the transcription from DNA. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 79: 1324–1335, 2001

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This article was published in Journal of Applied Polymer Science and referenced in Hydrology: Current Research

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