alexa Antibacterial activity and toxicological evaluation of semi purified hexane extract of Urtica dioica leaves.
Agri and Aquaculture

Agri and Aquaculture

Journal of Fertilizers & Pesticides

Author(s): Dr Rambir Singh, SABZAR AHMAD DAR, Dr Poonam Sharma

Abstract Share this page

The present study describes the antibacterial activity, phytochemical profile and toxicological evaluation of Urtica dioica. U. dioica leaves were subjected to solvent extraction by hexane, ethyl acetate, chloroform, methanol and water. All the extracts were tested for antibacterial activity against five clinical isolates of Gram positive and Gram negative bacteria by disc diffusion method. Hexane extract showed good antibacterial activity against all the five bacterial strains, hence it was further purified using silica column chromatography and MIC of the semi purified fraction-B (FB) of hexane extract was determined by serial tube dilution method. FB showed MIC value of 31.25, 250, 7.81, 31.25 and 125µg/ml against Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi¬¬, Klebsiella pneumoniae and Shigella flexneri respectively. FB was subjected to GC-MS analysis in search of potent antibacterial compound(s). Neophytadiene (26.97%), butyl tetradecyl ester (9.53%), Dibutyl phthalate (7.45%), Bis(2-ethyl hexyl) maleate (8.80%) and 1,2- benzenedicarboxylic acid (9.89%) were the major constituents which may be responsible for the antibacterial activity of FB of U. dioica. Sub-acute oral toxicity of FB was carried out in wistar rats at doses of 100, 200, 400 and 800 mg/kg body weight (bw) to assess index of safety. Hematological and biochemical parametes showed that the FB was safe at tested concentrations.

  • To read the full article Visit
  • Open Access
This article was published in Research Journal of Medicinal Plant and referenced in Journal of Fertilizers & Pesticides

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]om

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords