alexa Antibiotic resistance in Salmonella Typhimurium associates with CRISPR sequence type.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Phylogenetics & Evolutionary Biology

Author(s): Dimarzio M, Shariat N, Kariyawasam S, Barrangou R, Dudley EG

Abstract Share this page

Abstract Salmonella enterica subspecies enterica serovar Typhimurium is a leading cause of foodborne salmonellosis in the United States. The number of antibiotic resistant isolates identified in humans is steadily increasing, suggesting that the spread of antibiotic resistant strains is a major threat to public health. S. Typhimurium is commonly identified in a wide range of animal hosts, food sources, and environments, but little is known about the factors mediating the spread of antibiotic resistance in this ecologically complex serovar. Previously, we developed a subtyping method, CRISPR-MVLST, which discriminates among strains of several common S. enterica serovars. Here, CRISPR-MVLST identified 22 sequence types within a collection of 76 S. Typhimurium isolates from a variety of animal sources throughout central Pennsylvania. Six of the sequence types were identified in more than one isolate, and we observed statistically significant differences in resistance among these sequence types to 7 antibiotics commonly used in veterinary and human medicine, such as ceftiofur and ampicillin (p<0.05). Importantly, five of these sequence types were subsequently identified in human clinical isolates, and a subset of these isolates had identical antibiotic resistance patterns, suggesting that these subpopulations are transmitting through the food system. Therefore, CRISPR-MVLST is a promising subtyping method for monitoring the farm to fork spread of antibiotic resistance in S. Typhimurium.
This article was published in Antimicrob Agents Chemother and referenced in Journal of Phylogenetics & Evolutionary Biology

Relevant Expert PPTs

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords