alexa Anticancer and antiinflammatory activities of cucurbitacins from Cucurbita andreana.
Pharmaceutical Sciences

Pharmaceutical Sciences

Journal of Bioequivalence & Bioavailability

Author(s): Jayaprakasam B, Seeram NP, Nair MG

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Abstract Bioassay-guided purification of an extract of Cucurbita andreana fruits yielded cucurbitacins B (1), D (2), E (3), and I (4). These cucurbitacins were evaluated for their inhibitory effects on the growth of human colon (HCT-116), breast (MCF-7), lung (NCI-H460), and central nervous system (CNS) (SF-268) cancer cell lines, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) enzymes and on lipid peroxidation. Inhibitory activities of cucurbitacins B (1), D (2), E (3) and I (4), respectively, were for colon 81.5, 80.4, 77, and 65\% at 0.4 microM, breast 87, 78, 66.5, and 12\% at 0.4 microM, lung 96, 43, 37 and 2\% at 0.1 microM and CNS 92, 25, 24 and 4\% at 0.05 microM. Adriamycin (doxorubicin) was used as a positive control, which showed 64, 47, 45 and 71\% inhibition of HCT-116 (colon), MCF-7 (breast), NCI-H460 (lung) and SF-268 (CNS) cell lines, respectively, at 0.3 x 10(-5) M. Compounds 1, 2, 3, and 4 inhibited the COX-2 enzyme by 32, 29, 35, and 27\%, respectively, at 100 microg/ml. However these compounds did not inhibit the COX-1 enzyme at this concentration. Ibuprofen, naproxen and vioxx, commercial antiinflammatory drugs, were tested as controls for the inhibition of COX-1 and COX-2 enzymes at concentrations of 2.1, 2.5 and 1.67 microg/ml, respectively. Ibuprofen and naproxen exhibited 59 and 95\% COX-1, and 53 and 79\% COX-2 inhibitory activities, respectively. Vioxx showed specific COX-2 inhibition by 71\%. Also, cucurbitacins 1 and 4 inhibited lipid peroxidation by 59 and 23\%, respectively, at 100 microg/ml.
This article was published in Cancer Lett and referenced in Journal of Bioequivalence & Bioavailability

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