Author(s): Mahendra Srivastava
Ongoing programme in our Institute for the screening of extracts from marine flora and fauna of the Indian coasts , we noticed a promising antidiarrhoeal effect in the extract of Xylocarpus granatum mesocarp of the fruits which prompted us to take detailed study of this mangrove for its antidiarrhoeal effect.Methods: The fruits of the plant were collected from the Andaman coast of India. The mesocarp were separated, air dried and extracted with 50% aqueous ethanol at room temperature, the ethanol extract was fractionated into methanol soluble and methanol insoluble fractions, The active methanol fraction was further fractionated into chloroform soluble and insolubles. On purification of the active chloroform fraction 4 compounds were purified and bioassayed for their antidiarrhoeal effect by the standard methods of bioassay of the antidiarrheal activity.Results : The 50% aqueous ethanol extract of the mesocarp of the fruits given orally at a dose of 500mg/kg showed significant antidiarrhoeal activity in castor oil induced diarrhea model in rats. Among the fractions tested the chloroform soluble fraction showed promising activity at 250 mg/kg dose level. On further purification of chloroform fraction 4 compounds purified and were tested for antidiarrhoeal activity in rats. Conclusion:Three pure compounds showed promisingactivity. Further structure modification required to enhance the antidiarrhoeal effect.