Author(s): Boligon AA, Pereira RP, Feltrin AC, Machado MM, Janovik V,
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Abstract This study evaluated the antioxidant activities in the leaves and stem bark fractions of Scutia buxifolia. Cerebral lipid peroxidation (TBARS) was induced by Fe(II) and radical-scavenging activity was determined by DPPH method. Folin-Ciocalteu was used to determine phenolic contents. Quercetin, quercitrin, isoquercitrin and rutin were isolated from leaf ethyl acetate fraction and their levels were measured by high performance liquid chromatography-photodiode array detector. IC(50) (DPPH) varied from 4.35+/-1.30 to 29.55+/-0.54 microg/mL for stem bark and from 6.50+/-0.40 to 30.54+/-1.14 in the leaves. Ethyl acetate and butanolic fractions caused a sharp fall in TBARS production with IC(50) from 2.93+/-2.17 to 40.46+/-2.51 microg/mL for the leaves and 0.66+/-0.17 to 27.3+/-1.23 for the stem bark. Results obtained indicated that S. buxifolia has a great potential to prevent disease caused by the overproduction of free radicals and also it might be used as a potential source of natural antioxidant agents.
This article was published in Bioresour Technol
and referenced in Journal of Proteomics & Bioinformatics