Author(s): Arora DS, Chandra P
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Abstract The antioxidant activity of Aspergillus fumigatus was assayed by different procedures and correlated with its extracellular total phenolic contents. Different physio-chemical parameters were optimized to enhance the activity. The culture grown under stationary conditions for 10 days at 25°C at pH 7 gave the best antioxidant activity. Statistical approaches demonstrated sucrose and NaNO(3) to be the most suitable carbon and nitrogen sources, respectively. Response surface analysis showed 5\% sucrose, 0.05\% NaNO(3), and incubation temperature of 35°C to be the optimal conditions for best expression of antioxidant activity. Under these conditions, the antioxidant potential assayed through different procedures was 89.8\%, 70.1\%, and 70.2\% scavenging effect for DPPH radical, ferrous ion and nitric oxide ion, respectively. The reducing power showed an absorbance of 1.0 and FRAP assay revealed the activity of 60.5\%. Extracellular total phenolic content and antioxidant activity as assayed by different procedures positively correlated.
This article was published in ISRN Pharmacol
and referenced in Journal of Microbial & Biochemical Technology