alexa Antioxidant Effects of Vitamins C and E on Phenylhydrazine-Induced Haemolysis in Sprague Dawley Rats: Evidence for A better Protection by Vitamin E.
Toxicology

Toxicology

Journal of Clinical Toxicology

Author(s): Odigie IP, Okpoko FB, Ojobo PD, Odigie IP, Okpoko FB, Ojobo PD, Odigie IP, Okpoko FB, Ojobo PD, Odigie IP, Okpoko FB, Ojobo PD

Abstract Share this page

Abstract BACKGROUND: Phenyldrazine (PHDZ) provokes acute haemolysis and generates free radicals that are known to be involved in the toxicity of the drug. Vitamin C and E have been ascribed beneficial roles in many acute haemolytic states and oxidative stress. OBJECTIVE: The present study was designed to assess the antioxidant effect of vitamin C and E on PHDZ- induced haemolysis in SD rat in-vivo and ex-vivo. METHOD: Rats treated for weeks with oral vitamin E (500mg/kg/day, n=6), oral vitamin C (100mg/kg/day, n=6) and control rats, which received tap water; were monitored for peripheral blood parameters and haemolytic activity of erythrocytes at Baseline, 48 hours and 1 week respectively after intraperitonial administration of PHDZ (15mg/kg/day for 7 days). Osmotic fragility as well as organ weights were determined 1 week after PHDZ. Dose-dependent effects of vitamins C and E on haemolysis were determined at the end of 120 min incubation of rat RBC with the vitamins in phosphate-buffered-saline. RESULTS: Hb and PCV were better preserved in rats pre-treated with the antioxidants. The spleenomegaly, cardiomegaly and hepatomegaly associated with PHDZ treatment were significantly reduced in antioxidant pre-treated rats (P<0.001). reduction in cardiac weight was more marked in vitamin E group (0.56+/- 0.02g vs. 0.72+/-0.03g; n=6; P<0.01; vitamin E vs. PHDZ) compared to vitamin C (0.69+/-0.02g vs. 0.72+/-0.03g; n=6; P>0.05; vitamin C compared to PHDZ). RBC of vitamin E group exhibited better resistance to osmoticlysis (P<0.001). at higher doses (400-500mg/L), vitamin C caused significant haemolysis both in the absence (P<0.001) and presence of PHDZ (P<0.001). Vitamin E abolished the long tail of the osmotic fragiligram. CONCLUSION: The result of this study suggests that compared to vitamin C, vitamin E may better protect RBC against haemolysis induced by oxidative stress.
This article was published in Niger Postgrad Med J and referenced in Journal of Clinical Toxicology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords