alexa Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight mass spectrometry to metabolic phenotyping of natural variants in rice.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Kusano M, Fukushima A, Kobayashi M, Hayashi N, Jonsson P,

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Abstract We have developed a comprehensive method combining analytical techniques of one-dimensional (1D) and two-dimensional (GC x GC) gas chromatography-time-of-flight (TOF)-mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining 1D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs. This article was published in J Chromatogr B Analyt Technol Biomed Life Sci and referenced in Journal of Microbial & Biochemical Technology

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