Author(s): WietechaPosuszny R, Woniakiewicz M, Garbacik A, Chsy P, Kocielniak P
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Abstract The aim of this research was to develop, optimize and validate a modern and rapid method of preparation of human hair samples using microwave irradiation for the purpose of determination of six benzodiazepines: alprazolam, estazolam, lorazepam, clonazepam, diazepam and tetrazepam, with lormetazepam as an internal standard (IS). Extracts were analyzed ultrahigh performance liquid chromatography with mass spectrometry time of flight detection (UHPLC-MS-TOF). Optimal parameters of microwave-assisted extraction (type of extraction solvent, extraction temperature, extraction time and pH of borate buffer) for these drugs in spiked hair samples were defined. The limit of detection ((0.003-0.025 ng/mg), intra- (1.5-4.3\%) and interday (2.3-8.3\%) precision (CV) and accuracy of the assay (89.8-105.7\%) at three concentration levels: 0.22, 2.22 and 5.56 ng/mg were calculated. The optimized and validated MAE/UHPLC-MS-TOF method was then applied for analysis of clinical hair sample. Copyright © 2013 Elsevier B.V. All rights reserved.
This article was published in J Chromatogr A
and referenced in Pharmaceutica Analytica Acta