alexa Application of temperature-controlled attenuated total reflectance-mid-infrared (ATR-MIR) spectroscopy for rapid estimation of butter adulteration


Journal of Food & Industrial Microbiology

Author(s): N Kocaa, LE RodriguezSaona, WJ Harpera, NA KocaogluVurmaa

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There is a continual need for development of rapid methods that meet or exceed the detection levels of currently available analytical methods for authentication of food products. The objective of this study was to evaluate temperature-controlled attenuated total reflectance-mid-infrared (ATR-MIR) spectroscopy combined with multivariate analysis as a simple and rapid method for the determination of butter adulteration as a dairy food system. Commercial samples of butter fat were adulterated with margarine fat at levels ranging from 0% to 100% (v/v). Partial least square regression (PLSR) models gave standard error of cross-validation (SECV) of <1.2% (v/v) and correlation coefficients (r) > 0.99. Excellent predicting capabilities were obtained using an external validation set consisting of butter adulterated with margarines at ratios of 2.5%, 13%, and 45%. We have demonstrated the feasibility of a temperature-controlled ATR-MIR spectroscopy technique that would allow for rapid analysis of dairy products.

This article was published in Food Chemistry and referenced in Journal of Food & Industrial Microbiology

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