alexa Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Helliwell CA, Poole A, Peacock WJ, Dennis ES

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Abstract The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.
This article was published in Plant Physiol and referenced in Journal of Bioremediation & Biodegradation

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