alexa Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions.


Industrial Engineering & Management

Author(s): Beyer WF Jr, Fridovich I

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Abstract Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
This article was published in Anal Biochem and referenced in Industrial Engineering & Management

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