Author(s): Beyer WF Jr, Fridovich I
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Abstract Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
This article was published in Anal Biochem
and referenced in Industrial Engineering & Management