alexa Assessing analytical specificity in quantitative analysis using tandem mass spectrometry.


Neurochemistry & Neuropharmacology

Author(s): Kushnir MM, Rockwood AL, Nelson GJ, Yue B, Urry FM, Kushnir MM, Rockwood AL, Nelson GJ, Yue B, Urry FM, Kushnir MM, Rockwood AL, Nelson GJ, Yue B, Urry FM, Kushnir MM, Rockwood AL, Nelson GJ, Yue B, Urry FM

Abstract Share this page

Abstract OBJECTIVES: The necessity of confirmation of compound identity in quantitative analysis is well recognized for methods utilizing single mass spectrometry detection but is not commonly addressed for applications utilizing multiple-stage mass spectrometry (MSn). For MSn detection, no commonly accepted rules for assessment of analytical specificity in quantitative analyses have been established to date. METHODS: To assure compound identity, we evaluated approaches based on monitoring multiple mass transitions of a target compound followed by comparison of the branching ratios of the mass transitions. RESULTS: Monitoring multiple mass transitions along with evaluation of the ratio of their relative intensities allows the analyst to distinguish the target analyte from interferences in quantitative analysis. The strategy and the acceptance criteria are compound and method specific and should be established during the method development and validation. CONCLUSIONS: The certainty of analyte identity is very important in quantitative analysis using MSn detection; methods to verify analyte identity should be used in all critical applications. This article was published in Clin Biochem and referenced in Neurochemistry & Neuropharmacology

Relevant Expert PPTs

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version