alexa Assessment of oxidative stress in sperm and semen.


Journal of Addiction Research & Therapy

Author(s): Kashou AH, Sharma R, Agarwal A

Abstract Share this page

Abstract The chemiluminescence method is the most commonly employed technique as a direct measurement of reactive oxygen species (ROS) generation by spermatozoa. This assay is capable of quantifying both intracellular and extracellular ROS. Moreover, the use of various probes allows for differentiation between superoxide and hydrogen peroxide production by spermatozoa. When the total antioxidant reserves are overwhelmed by excessive production of ROS, it results in oxidative stress. Therefore correct measurement of both ROS and total antioxidant capacity (TAC) is essential in the assessment of oxidative stress in sperm and semen. This chapter describes the methodological approach for measuring seminal oxidative stress through the use of chemiluminescence assay for accurate measurement of ROS and the colorimetric assay for measurement of TAC. This article was published in Methods Mol Biol and referenced in Journal of Addiction Research & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version