alexa Autoantibodies against neonatal heart M1 muscarinic acetylcholine receptor in children with congenital heart block.
Dentistry

Dentistry

Dentistry

Author(s): Borda E, SterinBorda L

Abstract Share this page

Abstract Isolated congenital heart block may be associated with autoimmune disorder such as Sjögren Syndrome and systemic lupus erythematosus. In this work we demonstrate circulating autoantibodies against neonatal heart M1 muscarinic acetylcholine receptor (mAChR) in the sera of children with congenital heart block. This antibody were able to react with the second extracellular loop of the human M1 mAChR as demonstrated using a synthetic peptide in enzyme immune assay and binding assay. Affinity purified anti-peptide IgG as well as total IgG from children with congenital heart block, interfered with the specific radioligand occupancy from neonatal heart M1 mAChR, interacting irreversibly. The antipeptide antibodies also displayed an 'agonist-like' activity, i.e. decreased contractility, activated nitric oxide synthase activity and increased production of cyclic GMP. All of these effects were selectively blunted by pirenzepine and neutralized by the synthetic M1 peptide. Both binding and biological effects were obtained using neonatal rat heart instead adult heart and were independent of Ro/SS-A and La/SS-B antibodies and were also absent in the sera of normal children. A clinical relevance of these findings is demonstrated by a strong association between the existence of circulating M1 mAChR antipeptide antibodies and the presence of isolated congenital heart block, making these antibodies a proper marker of this disease. Copyright 2001 Academic Press. This article was published in J Autoimmun and referenced in Dentistry

Relevant Expert PPTs

Relevant Speaker PPTs

  • Eva E Avila
    Interaction of the parasite Trichomonas vaginalis with human neutrophil extracellular traps
    PPT Version | PDF Version
  • Amina Dahmani
    TRANSFORMING GROWTH FACTOR-β PROGRAMS CENTRAL-MEMORY DIFFERENTIATION IN EX-VIVO STIMULATED HUMAN T CELLS BY MODULATING ID3 EXPRESSION
    PPT Version | PDF Version
  • Eltayeb Tayrab
    Human mercury exposure associated with artisanal gold miners in Sudan
    PPT Version | PDF Version
  • Peter S. Nyasulu
    Prevalence and risk factors associated with acquisition of Sexually Transmitted Infections among people living with Human Immunodeficiency Virus in Diepsloot settlement, Johannesburg, South Africa
    PPT Version | PDF Version
  • Moorkath Nandakumaran
    Hyperglycemia alters maternal-fetal transport kinetics of manganese, chromium and vanadium in diabetic model human placental lobule in vitro : Implications for diabetes mellitu
    PPT Version | PDF Version
  • Carolina Alonso-González
    Melatonin sensitizes human breast cancer cells to ionizing radiation
    PPT Version | PDF Version
  • Jim Polarine
    Case studies of human fl ora and spore contamination in clean rooms
    PPT Version | PDF Version
  • Rune Thorbjørn Nordvang
    A Rational Approach to Identification of Wild Type Trans-sialidases for the Production of Human Milk Oligosaccharides
    PPT Version | PDF Version
  • Xueli Li
    Glycomics Profile Analysis by MALDI TOF/MS in Human CSF
    PPT Version | PDF Version
  • Julian M Menter
    Histology of Normal Human Skin
    PPT Version | PDF Version
  • Monray Edward Williams
    Molecular validation of putative antimicrobial peptides for improved Human Immunodeficiency Virus diagnostics via HIV protein p24
    PPT Version | PDF Version
  • Rashid Ramazanzadeh
    Effect of ciprofloxacin and levofloxacin alone or in combination with N-acetylcysteine on biofilm-producing Enterococcus faecalis: Contaminated root canals of extracted human teeth
    PPT Version | PDF Version
  • Geeta Shroff
    Improved Epilepsy and Speech Outcomes with Human Embryonic Stem Cell Therapy in Children with Cerebral Palsy
    PPT Version | PDF Version
  • Yosef Yarden
    Classically, the 3’untranslated region (3’UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3’UTR alone, without all other elements in mRNA such as 5’UTR and coding region. The importance of independent 3’UTR RNA (referred as I3’UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3’UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3’UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3’UTR were important for its tumor suppression activity. Then, the C/EBP 3’UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3’UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3’UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990’s to 2000’s, world scientists found several 3’UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3’UTR regions, although the existence of their transcribed products as independent 3’UTR RNAs is still to be confirmed. Our studies indicate that the independent 3’UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
    PPT Version | PDF Version
  • Gloria Cuenca-Bescós
    Biodiversity, impact, vulnerability and adaptation in human evolution
    PPT Version | PDF Version

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords