alexa Autoantibodies to CD45 in systemic lupus erythematosus.


Journal of Clinical & Cellular Immunology

Author(s): Mamoune A, Saraux A, Delaunay JL, Le Goff P, Youinou P,

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Abstract Western blotting with U937 cell extracts as the substrate, and enzyme-linked immunosorbent assays (ELISA) with U937-, Jurkat- and Daudi cell-purified CD45 molecules were used to detect anti-CD45 reactivity in patients with systemic lupus erythematosus (SLE). By immunoblotting, 16 of 64 SLE sera were shown to be positive (25.0\%). In the ELISAs, 13 out of 18 SLE sera reacted with the target CD45. Of these, three were not detectable on the blot. Importantly, 12 of these ELISA-positive sera contained IgM and IgG auto-antibodies. Neuraminidase-treatment of U937-precipitated CD45 molecules enhanced the reactivity to most of the isoforms, indicating that the antibodies may bind to asialylated polysaccharides. Copyright 1998 Academic Press. This article was published in J Autoimmun and referenced in Journal of Clinical & Cellular Immunology

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