Author(s): Karasawa R, Ozaki S, Nishioka K, Kato T
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Abstract Anti-oxidative enzymes protect living bodies from various oxidative stresses. In the systemic autoimmune diseases, autoantibodies to oxidized molecules and to anti-oxidative enzymes have been reported. To promote understanding of the relationships between autoimmunity and oxidative stress, we here investigate whether autoimmunity to the anti-oxidative peroxiredoxin (Prxs) enzymes exists in patients with systemic autoimmune diseases. Specifically, we detected autoantibodies to recombinant Prx I and Prx IV respectively by ELISA and western blotting. Next, clinical parameters were compared between the anti-Prx I or IV-positive and-negative patients. We found that 33\% of the 92 patients with autoimmune diseases tested possessed autoantibodies to Prx I (57\% in systemic lupus erythematosus (SLE), 19\% in rheumatoid arthritis (RA), 5\% in Behçet disease, and 46\% in primary vasculitis syndrome). In contrast, autoantibodies to Prx IV were detected in only 17\% of the same patients. No significant correlation was found between occurrence of the two autoantibodies. Clinically, possession of anti-Prx I autoantibodies correlated with lower serum levels of CH50, C3, and C4. Taken together, our data demonstrate the existence of autoantibodies to Prxs for the first time. The autoantibodies to Prx I may be involved in the pathophysiology of systemic autoimmune diseases such as SLE and vasculitis.
This article was published in Microbiol Immunol
and referenced in Journal of Data Mining in Genomics & Proteomics