Author(s): Tessaro MJ, Soliman SS, Raizada MN
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Abstract A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.
This article was published in Appl Environ Microbiol
and referenced in Journal of Probiotics & Health