alexa Benzodiazepines and metabolites from biological fluids by liquid chromatography electrospray tandem mass spectrometry.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): MorrisKukoski CL, Schaff JE, Reda LJ

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Abstract Solid-phase extraction and liquid chromatography-tandem mass spectrometry are invaluable techniques for the determination of benzodiazepines and metabolites in biological matrices. The reason for using tandem mass spectrometry is to increase limits of detection without the need for chemical derivatization. Here we describe a technique for the detection of 26 benzodiazepines and metabolites at a detection limit of approximately 1-2 ng/mL in blood and 1-5 ng/mL in urine when screened using a data-dependent scan method. This article was published in Methods Mol Biol and referenced in Pharmaceutica Analytica Acta

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