Author(s): Inborr J, Puhakka J, Bakker JG, Van der Meulen J
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Abstract Pigs were fed diets containing 40\% wheat bran incubated with a water: acetic acid mixture (control C) and a cellulase (Cel-i) or xylanase (Xyl-i) preparation or with addition of the cellulase (Cel-a) or xylanase (Xyl-a) preparation immediately before feeding. Stomach and ileal samples were analysed for xylanase and beta-glucanase activities by a standard method based on release of reducing substances and a modified method in which the actual pH of the sample was used. After feeding Cel-treated diets xylanase and beta-glucanase activities were higher in the stomach up to 4 hours after feeding. In the stomach the modified method gave significantly lower xylanase activities than the standard method. Ileal xylanase activity was significantly higher 2-6 hours after feeding Xyl-treated diets and ileal beta-glucanase activity was higher 4-6 hours after feeding Cel-treated diets. In ileal samples, the modified method gave significantly higher xylanase and lower beta-glucanase activities than the standard method. It is concluded that xylanase and beta-glucanase activities can be measured in digesta samples with the methods employed and that the added enzymes were largely inactivated between the feedings. Measuring digesta enzyme activities in conditions (pH) similar to the ones in the gastrointestinal tract may give a more reliable picture of the actual activity of added enzymes.
This article was published in Arch Tierernahr
and referenced in Journal of Bioprocessing & Biotechniques