alexa Bicarbonate increases tumor pH and inhibits spontaneous metastases.
Genetics

Genetics

Single Cell Biology

Author(s): Robey IF, Baggett BK, Kirkpatrick ND, Roe DJ, Dosescu J, , Robey IF, Baggett BK, Kirkpatrick ND, Roe DJ, Dosescu J,

Abstract Share this page

Abstract The external pH of solid tumors is acidic as a consequence of increased metabolism of glucose and poor perfusion. Acid pH has been shown to stimulate tumor cell invasion and metastasis in vitro and in cells before tail vein injection in vivo. The present study investigates whether inhibition of this tumor acidity will reduce the incidence of in vivo metastases. Here, we show that oral NaHCO(3) selectively increased the pH of tumors and reduced the formation of spontaneous metastases in mouse models of metastatic breast cancer. This treatment regimen was shown to significantly increase the extracellular pH, but not the intracellular pH, of tumors by (31)P magnetic resonance spectroscopy and the export of acid from growing tumors by fluorescence microscopy of tumors grown in window chambers. NaHCO(3) therapy also reduced the rate of lymph node involvement, yet did not affect the levels of circulating tumor cells, suggesting that reduced organ metastases were not due to increased intravasation. In contrast, NaHCO(3) therapy significantly reduced the formation of hepatic metastases following intrasplenic injection, suggesting that it did inhibit extravasation and colonization. In tail vein injections of alternative cancer models, bicarbonate had mixed results, inhibiting the formation of metastases from PC3M prostate cancer cells, but not those of B16 melanoma. Although the mechanism of this therapy is not known with certainty, low pH was shown to increase the release of active cathepsin B, an important matrix remodeling protease.
This article was published in Cancer Res and referenced in Single Cell Biology

Relevant Expert PPTs

Relevant Speaker PPTs

  • Chuanling Zhang
    Genetic Code Expansion in Natural Propagation for Site-Specific Engineering and Tracking of Single Adeno-Associated Viruses
    PPT Version | PDF Version
  • S. Sarker
    Tracking of viral evolution during an outbreak of emerging beak and feather disease virus (BFDV) infection in the critically endangered Orange-bellied parrot (Neophema chrysogaster)
    PPT Version | PDF Version
  • Bhanu Duggal
    Assessment of regional & global myocardial systolic function by 2D longitudinal speckle tracking in elderly patients with normal LV function
    PDF Version

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords