Author(s): Ali MS, Ravikumar S, Beula JM
Abstract Share this page
Abstract OBJECTIVE: To identify the larvicidal activity of the seagrass extracts. METHODS: Seagrass extracts, Syringodium isoetifolium (S. isoetifolium), Cymodocea serrulata and Halophila beccarii, were dissolved in DMSO to prepare a graded series of concentration. Batches of 25 early 4th instars larvae of Aedes aegypti (Ae. aegypti) were transferred to 250 mL enamel bowl containing 199 mL of distilled water and 1 mL of plant extracts (0.01 mg - 0.1 mg). After 24 h the mortality rate was identified with the formulae [(\% of test mortality - \% of control mortality)/(100 - \% of control mortality)] × 100. Each experiment was conducted with three replicates and a concurrent control group. A control group consisted of 1 mL of DMSO and 199 mL of distilled water only. RESULTS: : The root extract of S. isoetifolium showed maximum larvicidal activity with minimum concentration of extract of LC50= 0.0 604 ± 0.0 040)µg/mL with lower confidence limit (LCL) - upper confidence limit (UCL) = (0.051-0.071) and LC90=0.0 972µg/mL followed by leaf extract of S. isoetifolium showed LC50= (0.062 ± 0.005)µg/mL. The regression equation of root and leaf extract of S. isoetifolium for 4th instar larvae were Y= 4.909 + 1.32x (R(2)= 0.909) and Y= 2.066 + 1.21x (R(2) =0.897) respectively. The results of the preliminary phytochemical constituents shows the presence of saponin, steroids, terpenoid, phenols, protein and sugars. CONCLUSIONS: From the present study the ethanolic extracts of seagrass of S. isoetifolium possesses lead compound for development of larvicidal activity.
This article was published in Asian Pac J Trop Biomed
and referenced in Journal of Nanomedicine & Nanotechnology