Author(s): Samanta P, Pal S, Mukherjee AK, Senapati T, Ghosh AR
Freshwater teleostean fishes Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus were exposed to almix (66.67 mg/l) for a period of 30 days to investigate the toxicological responses on acetylcholinesterase (AChE), oxidative and antioxidant enzymes. AChE activity was increased significantly (p< 0.05) in muscle, brain and spinal cord in all test fishes, highest in muscle of H. fossilis but lowest in spinal cord of A. testudineus. Significant increased lipid peroxidation (LPO) level in all tissues was observed after almix intoxication, maximum in liver of O. niloticus and minimum in brain of H. fossilis. Catalase (CAT) activity was increased significantly in all fish tissues after almix exposure while glutathione-S-transferase (GST) showed significant reduced activity in liver, in particular, maximum in liver of H. fossilis. Protein content in all concerned tissues was significantly declined and highest reduction was observed in muscle of A. testudineus while lowest in liver of H. fossilis. So, the present study is able to shed light on the changes in acetylcholinesterase activity, oxidative parameters and antioxidant enzyme profile under long-term exposure of almix and these responses can be used as indicators of herbicidal contamination.