alexa Biodegradation of atrazine and related s-triazine compounds: from enzymes to field studies.
Agri and Aquaculture

Agri and Aquaculture

Journal of Aquaculture Research & Development

Author(s): Wackett LP, Sadowsky MJ, Martinez B, Shapir N

Abstract Share this page

Abstract s-Triazine ring compounds are common industrial chemicals: pesticides, resin intermediates, dyes, and explosives. The fate of these compounds in the environment is directly correlated with the ability of microbes to metabolize them. Microbes metabolize melamine and the triazine herbicides such as atrazine via enzyme-catalyzed hydrolysis reactions. Hydrolytic removal of substituents on the s-triazine ring is catalyzed by enzymes from the amidohydrolase superfamily and yields cyanuric acid as an intermediate. Cyanuric acid is hydrolytically processed to yield 3 mol each of ammonia and carbon dioxide. In those cases studied, the genes underlying the hydrolytic reactions are localized to large catabolic plasmids. One such plasmid, pADP-1 from Pseudomonas sp. ADP, has been completely sequenced and contains the genes for atrazine catabolism. Insertion sequence elements play a role in constructing different atrazine catabolic plasmids in different bacteria. Atrazine chlorohydrolase has been purified to homogeneity from two sources. Recombinant Escherichia coli strains expressing atrazine chlorohydrolase have been constructed and chemically cross-linked to generate catalytic particles used for atrazine remediation in soil. The method was used for cleaning up a spill of 1,000 pounds of atrazine to attain a level of herbicide acceptable to regulatory agencies.
This article was published in Appl Microbiol Biotechnol and referenced in Journal of Aquaculture Research & Development

Relevant Expert PPTs

Relevant Speaker PPTs

  • Rohin Vinayak
    Role of compassion fatigue in quality of life, and marital satisfaction among spouses of patients with diabetes type 2
    PPT Version | PDF Version
  • Saraswathi K
    Diagnosis of different stages of non-proliferative diabetic retinopathy
    PPT Version | PDF Version
  • Zuheir Barsoum
    Zuheir-Barsoum-Khalifa-University-UAE-Life-extension-upgrade-and-repair-of-welded-structures-Towards-the-use-of-High-Strength-Steels
    PPT Version | PDF Version
  • Mikael Bjerg Caspersen
    Innovative albumin based technology for half-life extension and optimization of Biotherapeutics
    PPT Version | PDF Version
  • Yosef Yarden
    Classically, the 3’untranslated region (3’UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3’UTR alone, without all other elements in mRNA such as 5’UTR and coding region. The importance of independent 3’UTR RNA (referred as I3’UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3’UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3’UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3’UTR were important for its tumor suppression activity. Then, the C/EBP 3’UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3’UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3’UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990’s to 2000’s, world scientists found several 3’UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3’UTR regions, although the existence of their transcribed products as independent 3’UTR RNAs is still to be confirmed. Our studies indicate that the independent 3’UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
    PPT Version | PDF Version
  • Jihea Choi
    Health-related Quality of Life of Adolescent with Idiopathic Scoliosis in Korea
    PPT Version | PDF Version
  • Raquel García Pacheco
    Evaluation of the ppm-h concept for end-of-life RO membrane into recycled NF and UF membranes
    PPT Version | PDF Version
  • Ana de Guzmán Báez
    Gypsum to Gypsum (GtoG): The European life project that aims to transform the gypsum waste market
    PPT Version | PDF Version
  • Tahany H. Ayaad
    External immune stimulation and its impact on the honey bee Apis mellifera jementica in Saudi Arabia
    PPT Version | PDF Version
  • S Bhuvaneswari
    Effect of packaging film of different thicknesses on shelf life and quality of minimally processed onion
    PPT Version | PDF Version
  • Mehmet Saydam
    Quality-of- life, body image and cosmesis after single incision laparoscopic cholecystectomy versus laparoscopic cholecystectomy
    PPT Version | PDF Version
  • Tina Abrefa Gyan
    Socio-demographic factors, social support, and quality of life of people living with HIV/AIDS in Ghana
    PPT Version | PDF Version
  • Antonio Cilla
    Antiproliferative effect of main dietary phytosterols and/or β-cryptoxanthin in human colon cancer Caco-2 cells through cytosolic Ca2+ - and oxidative stress-induced apoptosis
    PPT Version | PDF Version
  • Tamer I Mahmoud
    Early Life Intervention Diminishes Manifestations of Sjögren's Syndrome in NOD.H-2h4 mice
    PPT Version | PDF Version
  • S P Goyal
    Genetic structure of tiger populations in India and its significance in forensics
    PPT Version | PDF Version

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords