Author(s): Sharifi Tehrani A, Ramezani M
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Abstract Fusarium wilt caused by Fusarium oxysporum Sch. is one of the most important diseases of onion in Iran. Application of chemicals especially as soil drench, increased cost of onion production and may be dangerous for environment. One of the effective techniques to suppress soil-born diseases in biological control with antagonistic rhizobacteria. Experiment were carried out with 120 bacterial isolates that were collected from onion rhizosphere. Six highly effective isolates were selected from these antagonists for subsequent studies. These strains were used to investigate their biocontrol traits in vitro and their ability to suppress the onion wilt in vivo (soil and seed treatments). According to the biochemical, physiological and morphological test, the isolates 22, 38, 46 and 52 were identified as Bacillus spp. The isolates 16 and 48 were identified as Pseudomonas fluorescens. The isolates of Bacillus spp. produced volatile metabolites that inhibited mycelia growth of Fusarium oxysporum. In soil treatment, the isolates 22 and 52 with 56\% and 51\% had the highest effect in reducing the Fusarium wilt of onion. The mixture of two isolates reduced 60\% the disease. In seed treatment the isolate 22 with 41\% had the greatest effect on reducing the onion Fusarium wilt.
This article was published in Commun Agric Appl Biol Sci
and referenced in Journal of Addiction Research & Therapy