alexa Biological significance of unwinding capability of nuclear matrix-associating DNAs.
Molecular Biology

Molecular Biology

Journal of Cell Science & Therapy

Author(s): Bode J, Kohwi Y, Dickinson L, Joh T, Klehr D,

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Abstract Matrix attachment regions (MARs) are thought to separate chromatin into topologically constrained loop domains. A MAR located 5' of the human beta-interferon gene becomes stably base-unpaired under superhelical strain, as do the MARs flanking the immunoglobulin heavy chain gene enhancer; in both cases a nucleation site exists for DNA unwinding. Concatemerized oligonucleotides containing the unwinding nucleation site exhibited a strong affinity for the nuclear scaffold and augmented SV40 promoter activity in stable transformants. Mutated concatemerized oligonucleotides resisted unwinding, showed weak affinity for the nuclear scaffold, and did not enhance promoter activity. These results suggest that the DNA feature capable of relieving superhelical strain is important for MAR functions.
This article was published in Science and referenced in Journal of Cell Science & Therapy

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