alexa Bioorthogonal chemical tagging of protein cholesterylation in living cells.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Heal WP, Jovanovic B, Bessin S, Wright MH, Magee AI,

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Abstract We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins. This article was published in Chem Commun (Camb) and referenced in Journal of Glycomics & Lipidomics

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