alexa Biosynthesis and characterization of 3-hydroxyalkanoate terpolyesters with adjustable properties by Aeromonas hydrophila.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Zhang HF, Ma L, Wang ZH, Chen GQ

Abstract Share this page

Abstract Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and enoyl-CoA hydratase, and another its recombinant harboring phaAB encoding beta-ketothiolase and acetoacetyl-CoA reductase, respectively, when grown in lauric acid and/or valerate. The terpolyesters produced by A. hydrophila 4AK4 (phaAB) grown in velarate were found to produce copolymers P(3HB-co-3HV) containing high 3HV fractions with a maximum of 99 mol\% 3HV. In terpolyesters, 3HV ranged from 9 to 32 mol\% depending on the valerate concentration and strain used. A maximal terpolyester P(3HB-co-3HV-co-3HHx) content in dry cells was 71 wt\%. Transmission electron microscopy study of A. hydrophila 4AK4 harboring phaPCJ revealed the full occupation of terpolyester P(3HB-co-3HV-co-HHx) in the cellular spaces. Terpolyesters with various monomer compositions showed changing thermal and mechanical properties. Those with higher 3HV fractions demonstrated an improved property over the lower HV containing ones. This article was published in Biotechnol Bioeng and referenced in Journal of Microbial & Biochemical Technology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords